David Jeruzalmi
Structural studies of nucleoprotein complexes that form at sites of DNA replication initiation

Accurate replication of DNA is mediated by multi-protein assemblies that initiate
DNA synthesis, elongate daughter strands and assure the integrity of the catalyzed chemical reactions. We are using X-ray crystallography to determine the structures of selected functionally relevant assemblies with the goal of understanding biochemical data present in these systems. Structure determinations are followed by functional studies of designated variants of these assemblies to test models of biochemical function .

Publications:
Jeruzalmi, D., Yurieva, O., Zhao, Y., Young, M., Stewart, J., Hingornai, M., O’Donnell, M. and Kuriyan, J. 2001. Mechanism of Processive Clamp Opening by the Delta Subunit Wrench of the Clam Loader Complex of E. coli DNA Polymerase III. Cell 106: 417-428.

Jeruzalmi D, O’Donnell M and Kuriyan J. 2001. Crystal Structure of the Processivity Clamp Loader Gamma (g) Complex of E. coli DNA Polymerase III. Cell 106: 429-441.